The scene has a girl talking about her sexuality or thoughts on being with a girl.
Series Page
Episode Title: Our Idiot Brian
Timestamp: 02:56-03:17
Rating: 1
Ruth comments on how she thinks Lois is multiple times.
“What about your hot mom, Meg?”
“You mom is so fucking hot…”
Series Page
Episode Title: Bigfat
Timestamp: 01:12-01:25
Rating: 2
In a deleted scene, it’s revealed that Francine from American Dad and Lois hooked up with each other in 1984 at a concert.
Francine then whispers to her: “You’ve ruined me for all men.”
Series Page
Episode Title: Baby, You Knock Me Out
Timestamp: 04:07-04:16
Rating: 1
When Lois gets forced into a boxing match with another woman, she jokingly suggests that they’ll pretend to hit each other and then kiss a little.
Series Page
Episode Title: Partial Terms of Endearment
Timestamp: 00:38-01:24
Rating: 1
At a college reunion, Lois admits to Peter that Naomi was the woman she experimented quite a bit with.
Series Page
Episode Title: Stew-Roids
Timestamp: 01:11-01:41
Rating: 3
Lois offers to rub lotion on Bonnie’s back during while they’re outside.
While doing so, Lois seems to go a little too low with touching Bonnie’s body and begins to get closer while smiling, implying she was enjoying it sexually.
Quagmire watches from behind a fence, but the scene cuts away before anything actually happens.
Series Page
Episode Title: You May Now Kiss the…Uh…Guy Who Receives
Timestamp: None (Deleted Scene)
Rating: 1
In a deleted scene, while Lois is talking to a priest about her views on homosexuality, she brings up her experience with lesbian sex.
Lois: “I’ve never had any problems with gays before. I’ve even had my own experiences and it is absolutely amazing. I mean girl-on-girl sex, it’s everything you would have imagined it would be.”
Priest: “Now let me ask you this, have you had any girl-on-girl sex recently?”
Lois: “Oh no no no, I mean for heaven’s sake, I’ve had 3 children.”
Series Page
Episode Title: Brian Sings and Swings
Timestamp: 14:43-15:10
Rating: 4
When Lois asks Meg to prove that she’s a lesbian by kissing her girlfriend, Sarah, by kissing her, so Meg gives her a quick peck on the cheek.
Lois then says that wasn’t a real kiss and demonstrates by making out with Sarah, leaving Sarah looking very pleased.
Series Page
Timestamp: 17:46-18:26
Rating: 3
Nana K. is briefly kissed by Nana O., causing Nana K. to question her sexualtiy.
Nana K. then tries to rationalize that since it was a girl, she wasn’t cheating on her boyfriend.
Series Page
Chapter Link
Rating: 5
A maid catches Irufa masturbating to a pair of Maya’s panties.
After getting caught, Irufa seduces and has lesbian sex with her.
Eventually, Irufa turns into a futanari and stays as one for the rest of the scene.
Series Page
Episode Link
Timestamps: 04:35-04:48 / 07:15-09:15
Rating: 2
During the fight, after Samus loses their armor and appears with the zero suit on, Master Chick/Nicole becomes visibly aroused with a spark at her crotch.
After the fight, the two of them lean in close and there is some romantic implications/tension.
I was unable to find a source for this confirmation, so take this with a grain of salt, but supposedly Monty (R.I.P.) confirmed that he chose to have Master Chick/Nicole not fight back after seeing Samus in her zero suit because she was smitten by her. (Honestly, that seems to be the case after the crotch shot lol.)